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| C. Ciências Biológicas - 8. Genética - 4. Genética Molecular |
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| COMPARISON BETWEEN Leishmania chagasi AND OTHERS Leishmania SPECIES |
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| Francisco Raniere Sales Menezes 2, Allan Rodrigo Soares Maia 2, Elton José Rosas de Vasconcelos 1, João José Simonni Gouveia 1, Marianna Colares de Albuquerque 1, Adriana Rocha Tomé 1, Diana Magalhães Oliveira 1, Raimundo Bezerra da Costa 1, Rodrigo Maggioni 1, 3 e Francisco Linhares Arruda Ferreira Gomes 1, 2 |
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| 1 - Núcleo de Genômica, Faculdade de Veterinária/UECE
2 - Coordenação do Curso de Ciências Biológicas/UECE
3 - Faculdade de Educação, Ciências e Letras do Sertão Central/UECE
, UNIVERSIDADE ESTADUAL DO CEARÁ - UECE |
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| The application of bioinformatic knowledge could provide new tools for the etiologic control of New World visceral leishmaniosis, a disease caused by the protozoa Leishmania chagasi. Complementary DNA (cDNA) was sequenced using the strategy of Expressed Sequence Tags analysis (EST). Our main goal was to compare the L. chagasi ESTs sequenced with information published elsewhere. The clusters were obtained through the sequencing of 2,096 cDNA, using the Genetic Analyzer ABI Prism 3100. The contigs quality was analyzed using the software PHRED and the nucleotides sequences were aligned against the NCBI database using the softwares BLAST. The homolog sequences were grouped into two categories: 1.via observation of the amount of homolog sequences between the L. chagasi and some specific chromosomes of the Leishmania major; 2. took into account the genes already known and published for the Leishmania genus. Ninety contigs came out after the cDNA clone sequencing. The sequence revealed a great similarity between the L. chagasi and the L. major species, containing 21 contigs allocated in chromosomes already known. Eight L. chagasi cDNA contigs presented perfect alignment with the chromosome 27 of L. major. The remaining sequences without significant homology were set aside as "no hit" sequence database. We can state that these contigs have the highest genetic similarity with L. major, since more than 70% of the sequences aligned preferentially with this protozoan compared to other species of this genus. The existence of perfect cDNA alignments of L. chagasi with genomic DNA from other species of the same genus reinforces the current belief that this protozoan contains a small number of introns. We anticipate that the data obtained through the analysis of the L. chagasi ESTs will open avenues for future molecular, phylogenetic and clinical studies. |
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Instituição de fomento: CNPq
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Trabalho de Iniciação Científica
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| Palavras-chave:
Leishmania chagasi; bioinformatics; leishmaniosis |
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Anais da 57ª Reunião Anual da SBPC - Fortaleza, CE - Julho/2005
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