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| C. Ciências Biológicas - 6. Farmacologia - 1. Farmacologia Bioquímica e Molecular | ||
| PRO- AND ANTI-INFLAMMAROTY ACTIVITIES OF THE LATEX FROM Calotropis procera ARE PRESENT IN DIFFERENT PROTEIN FRACTIONS | ||
| Jefferson Soares de Oliveira 1 (jefferson.oliveira@gmail.com), Michael Will de Lima 2, Rodney Oliveira Mesquita 2, Nylane Maria Nunes Alencar 4, Nathalie Ommundsen Pessoa 3 e Márcio Viana Ramos 5 | ||
| (1. Iniciação Científica, Curso de Ciências Biológicas, Universidade Federal do Ceará – UFC; 2. Iniciação Científica, Curso de Farmácia, Universidade Federal do Ceará – UFC; 3. Graduado, Curso de Ciências Biológicas, Universidade Federal do Ceará – UFC; 4. Prof. Dr. do Depto. de Fisiologia e Farmacologia, Universidade Federal do Ceará - UFC; 5. Prof. Dr. do Depto. de Bioquímica e Biologia Molecular, Universidade Federal do Ceará – UFC) | ||
| INTRODUÇÃO:
Latex from Calotropis procera (LCP) is well known for its medicinal as well as toxics properties. Experimentally it has been reported the occurrence of pro- and anti-inflammatory events in animals receiving latex doses. However, these studies were carried out with the whole latex. In this work the fresh latex from C. procera was fractioned and both pro- and anti-inflammatory activities were checked in the fractions. |
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| METODOLOGIA:
Male rats (150-250g body weight) were housed in a temperature-controlled room with free access to water and food. The C. procera latex was collected in distilled water to give a dilution of 1:2 (v:v) and centrifuged at 6.000 g during 10 min at 25ºC and the rubber-rich precipitate (ppt1) was stored. The supernatant was dialyzed against distilled water (1:1/v:v) during 1 hour and the water of the first hour of dialysis was lyophilized (DP). The non-dialyzable fraction was continuously dialyzed against distilled water and centrifuged again under the same conditions before. The new clear supernatant free-rubber (LP) was lyophilized and the precipitate containing rubber (ppt2) was joined to the previous one and lyophilized (BP). The fractions (DP, RP and LP) were administered: i.p. (2.5 mg/cavity) for non-treated animals or i.v. (2.5 mg/cavity) 30 minutes before injection of carrageenin i.p. (500mg/cavity). Neutrophil migration (NM) was evaluated 4h after injection of the latex fractions i.p. or Cg by counting the cells in the peritoneal fluids. The results were reported as mean + S.E.M of the number of neutrophil per microlitre of peritoneal wash. |
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| RESULTADOS:
The assay revealed that only DP i.p. (2.5 mg/cavity) caused Neutrophil migration [1142±265 (saline) and 4522±763 (DP)] and this fraction did not reverse the inflammation induced by carrageenin. On the other hand, Neutrophil migration induced by Cg was inhibited by LP and RP. The inhibition reached 69% and 72%, respectively. LP is a protein rich fraction as previously determined and DP was recently proven to contain proteins by analysis through SDS-PAGE. |
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| CONCLUSÕES:
The ensemble of results suggests that the inflammatory and anti-inflammatory effects of the LCP are promoted by different proteins fractions that can be easily fractionated apart. The purification and biochemical characterization of these molecules will be interesting to establish the LCP as an important tool in study and control of the inflammatory response. |
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| Instituição de fomento: CNPq/PADCT, FUNCAP, and IFS. | ||
| Trabalho de Iniciação Científica | ||
| Palavras-chave: Calotropis procera; Inflammation; Carrageenin. | ||
| Anais da 57ª Reunião Anual da SBPC - Fortaleza, CE - Julho/2005 |