| C. Ciências Biológicas - 6. Farmacologia - 1. Farmacologia Bioquímica e Molecular |
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DOSE-DEPENDENT INHIBITION ON CAMP CHICK RETINA CONTENT BY THE ROSEWOOD(Aniba rosaeodora Ducke) OIL |
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| Amanda Magno de Parijos 1 |
| José Guilherme S. Maia 2 |
| Lucia de Fatima Sobral Sampaio 1 |
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| (1. Departamento de Fisiologia/UFPA; 2. Departamento de Química/UFPA) |
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| INTRODUÇÃO: |
The rosewood oil (RO) (Aniba rosaeodora Ducke), rich in the monoterpene linalool, has been popularly implicated in the anticonvulsivant phytotherapeutical. In the chick retina the pure oil (1-3 ml/ml medium) inhibits maximally the adenosine-3´, 5´-monophosphate cyclic forskolin-stimulated, but presenting no effect on basal level, showing that these doses are out of toxicity range in this kind of tissue. The present aim is to investigate the RO half-maximal dose to inhibit the cAMP forskolin stimulated in chick retina. |
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| METODOLOGIA: |
The retinas from 2-3 days post-hatching chicks, in a medium containing the phosphodiesterase inhibitor 4-(3-butoxy-4-methoxy-benzyl)-imidazolidin-2-one (0.5x10-3 M), were incubated with RO 85% linalool-containing, diluted with tween 20 in a dose range of 0-3 ml of pure RO, contained in the dilution medium during 20 min before the forskolin (10-5 M) stimulation. The determination of cAMP accumulation was made by a competitive enzyme immunoassay system (Amersham Biosciences & GE Healthcare) |
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| RESULTADOS: |
The Tween 20 (20 ml/ml medium) alone did not modify the cAMP retinal content (N = 3). The RO dose response curve was adjusting by Boltzmann sigmoidal equation (R2 = 0.98), with a V50 = 0.04 ml/ml medium (Confidence intervals = 0.004-0.06 ml/ml). |
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| CONCLUSÕES: |
The results in comparison with other works that has showed the linalool inhibitory effect on glutamate binding in mammalian brain, is contributing to understand the action mode of the rich-linalool RO on Central Nervous System. |
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Trabalho de Iniciação Científica
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Palavras-chave: Linalool; AMPc; Retina. |
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| Anais da 58ª Reunião Anual da SBPC - Florianópolis, SC - Julho/2006 |