| C. Ciências Biológicas - 2. Biologia - 2. Biologia Evolutiva |
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BLOOD ANTIOXIDANT DEFENSES OF THE HAMMERHEAD SHARK Sphyrna lewini |
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| Danilo Wilhelm Filho 1 |
| Aline Pereira 1 |
| Patrícia Backes 1 |
| Leonilda Banki Maçao 1 |
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| (1. Universidade Federal de Santa Catarina) |
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| INTRODUÇÃO: |
The oxygen metabolism in aerobic organisms implies the formation of reactive oxygen species (ROS). These ROS can oxidize biologically relevant molecules leading to alterations in normal cell and tissue functions. This oxidation is functionally minimized by a fairly complex panoply defences, like antioxidant enzymes. Ulike other vertebrates, fish are exposed to daily or seasonal changes in both temperature and oxygen availability. Like other vertebrates, fish possess an ant defense system which utilizes enzymatic and non-enzymatic mechanisms. |
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| METODOLOGIA: |
Several parameters, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase activities, as well as TBARS contents was analyzed in palsma and erythrocytes in the hammerhead shark Sphyrna lewini. |
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| RESULTADOS: |
Superoxide dismutase activity was 0.546 U.ml-1, catalase was 9.36 mmol.min-1.ml-1, glutathione peroxidase was 0.96 mmol.min-1.ml-1, glutathione reductase was 0.35 mmol.min-1.ml-1 and glutathione-S-transferase was 0.59 mmol.min-1.ml-1. TBARS contents was 0.04 nmol.ml-1. |
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| CONCLUSÕES: |
The antioxidant levels found in the hammerhead shark were in accordance to the levels found in marine fish species. |
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Instituição de fomento: CNPQ
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Trabalho de Iniciação Científica
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Palavras-chave: Antioxidant Defences ; Oxidative Stress; Sphyrna lewini. |
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| Anais da 58ª Reunião Anual da SBPC - Florianópolis, SC - Julho/2006 |