62ª Reunião Anual da SBPC
C. Ciências Biológicas - 10. Microbiologia - 2. Microbiologia Aplicada
DETECTION OF SULFATE-REDUCING PROKARYOTES IN MANGROVE SEDIMENTS NEAR PETROLEUM FACILITIES IN NORTHEAST BRAZIL
Ciáxares Magalhães Carvalho 1
Bruno Gomes de Sousa 1
Paulo Sérgio Marinho Lúcio 1
Djalma Ribeiro da Silva 2
Carlos Alfredo Galindo Blaha 3
1. Departamento de Biologia Celular e Genética - UFRN
2. Prof. Dr./Orientador Núcleo de Estudos em Petróleo e Gás Natural - UFRN
3. Prof. Dr./Orientador - Departamento de Biologia Celular e Genética - UFRN
INTRODUÇÃO:
Sulfate-reducing prokaryotes (SRP) are responsible for petroleum damages, like souring, oil biodegradation and biocorrosion of metal surfaces in industrial facilities. The H2S production through SRP metabolism drives to serious risks to petroleum workers health due to its poisoning capability. SRP populations grow anaerobically in environments, but are subject to the influence of oil contamination in diverse ecosystems. The Potiguar Petroliferous Basin (PPB) comprise Rio Grande do Norte and Ceará States. The study of SRP diversity indigenous in mangrove sediment is remarkably important to determine the capabilities of response to eventual oil contamination in estuarine sites as well as in the petroliferous industry water supplies. More interestingly, the mangrove biome is a very critical and fragile interface of the ocean frontier. This work aims to monitor the occurrence of SRP in mangrove sediments of PPB using molecular bioprospection of metagenome obtained in a simulating crude oil contamination assay.
METODOLOGIA:
Mangrove sediment samples were collected in Areia Branca/RN, Fortim/CE and Paracuru/CE. Areia Branca and Paracuru are sites with intense activity by the oil industry. Fortim is a touristic place that is vulnerable to oil contamination. The analyses of the environmental samples were designed in microcosms that were contaminated with 3 % of crude oil. Additionally, there were un-contaminated microcosms. The metagenomic DNA was periodically extracted and showed in two cycles with progressive days of intervals: 0, 3, 7, 14, 28, 60, 63, 67, 74, 88, 120 days. The PCR oligonucleotides for dsrB gene was choose using bioinformatics tools from NCBI and manual edition. The PCR and PCR-DGGE analyses was performed as described in the literature.
RESULTADOS:
The metagenomic bioprospection of sulfate-reducing prokaryotes in PPB by dsrB biomarker on mangrove metagenomic DNA shows the occurrence in Areia Branca, Paracuru e Fortim localities. Considering the three sites, SRP were detected in several oil contaminated mangrove samples. Specifically in Areia Branca, more than 60% of samples contaminated or un-contaminated reveal the presence of SRP. The metagenome PCR-DGGE profiles from Paracuru and Fortim sites show fluctuation of the SRP communities according to the sampling periods and the crude oil presence. The interference of the petroleum industry in Areia Branca and Paracuru should be determinant to microbial diversity. The Fortim site do not have direct influence of petroleum facilities and the result displays a particular SRP profile, differing of the other two sites, where the results show SRP populations with an adaptation/tolerance or biodegradation capabilities to oil contamination.
CONCLUSÃO:
This work provides an important background about the SRP communities in estuarine sediments of PPB sites subject to a risk by oil contamination. The seasonal fluctuation of SRP communities suggests that these microorganisms are capable to survive in the presence of oil pollutants in the studied mangroves. The results suggest that indigenous SRP may play a role on the potential restore of contaminated mangroves.
Instituição de Fomento: National Council of Scientific and Technogical Development - CNPq
Palavras-chave: Sulfate-reducing prokaryotes, Oil contamination, Metagenomic bioprospection.