62ª Reunião Anual da SBPC
C. Ciências Biológicas - 9. Imunologia - 5. Imunologia
HUMAN LEUKOCYTE ANTIGEN-G (HLA-G) EXPRESSION GASTRIC SPECIMENS HARBORING THE HELICOBACTER PYLORI INFECTION AND GASTRIC CARCINOMA
Cláudia Nunes Oliveira 2
Eduardo Antônio Donadi 3
Janaína Cristiana de Oliveira Crispim 1
Catarina de Lourdes Gesteira Pedreira 1
Wenna Gleyce Araújo do Nascimento 1
Keyla Borges Ferreira Rocha 2
1. Department of Clinical and Toxicological Analysis - UFRN
2. Department of Patology - UFRN
3. Department of Clinical Medicine, Faculty of Medicine of Ribeirão Preto - USP
INTRODUÇÃO:
HLA-G, a nonclassical human leukocyte antigen (HLA) class I molecule, has been reported as a critical immunosuppressive molecule, this molecule was shown to be largely expressed at sites of inflammation and cancer. Although several studies reported association of HLA-G molecule involved in inflammatory diseases, cancer and viral infections yet nothing is known about the relationship of this molecule in bacterial infections, specifically those related to Helicobacter pylori, that has some types strains are known carcinogen. We investigated the expression HLA-G in gastric biopsies of patients with different degrees of lesion harboring H. pylori, stratifying patients according to the severity of gastric injury and clinical picture.
METODOLOGIA:
65 gastric specimens harboring H.pylori were evaluated, by immunohistochemistry, using the primary monoclonal antibody anti-HLA-G (EXBIO-Republic Czech), and stratified as carcinoma (19 cases) or gastric ulcers (34 cases). Other criteria were analyzed by staining of hematoxylin-eosin and Giemsa, such as index of inflammatory activity, intensity of H.pylori, presence of eosinophils, accumulation in lymphoid follicles, the chronicity, metaplasia, hyperplasia foveola, ulceration, cystic dilatation, atrophy, atypia of repair amendment.
RESULTADOS:
In the group as a whole, HLA-G molecules were detected in 53 cases (82%). Among specimens that presented HLA-G, 34 cases (54%) had inflammation and 19 cases (29%) carcinoma (p = 0, 0864). By observing the control group it was felt that this had no HLA-G, which shows there is meaning in the expression of this protein, when patients are affected by H. pylori.
CONCLUSÃO:
These results indicate that the presence of HLA-G is associated with a inflammatory activity and carcinoma in gastric specimens harboring H.pylori.
Palavras-chave: Helicobacter pylori, carcinoma, HLA-G.