63ª Reunião Anual da SBPC

E. Ciências Agrárias - 1. Agronomia - 4. Fitotecnia

Modifications in total soluble amino acids and proline of 14 genotypes of Phaseolus vulgaris

Francisca Daniela de Araújo Padilha 1 Argemiro Pereira Martins Filho 1 Eldenira Barbosa Uchôa 1 Talitha Soares Pereira 1 Izabelle Pereira Andrade 1 Allan Klynger da Silva Lobato 1

1. Núcleo de Pesquisa Vegetal Básica e Aplicada

INTRODUÇÃO:

Normally specie with agronomical potential when cultivated in field conditions can be affected by abiotic and biotic stresses, in which are characterized by environment and biological factors, respectively. Therefore, proline by to be an osmoregulator can be used as tool to measure the ability of higher plants in to attenuate inadequate situations. Study had aim to evaluate behavior of nitrogen compounds such as total soluble amino acids and proline in leaf of 14 genotypes of common bean.

METODOLOGIA:

Study was conducted in experimental area located in Centro de Treinamento em Irrigação (CTI) of Universidade Estadual de Maringá (UEM), during 2008 season. Fertilization was carried out based in soil analysis and crop exigency. Plants were grown in field conditions with minimum/maximum temperature of 15.2/32.7ºC, and relative humidity was 48/74%, respectively. Experimental design used was randomized blocks, with 14 genotypes (IAPAR 81, IPR Tangará, IPR Uirapuru, BRS Campeiro, CHC 9729, CHP 9859, GEN C2-1-1, GEN C2-1-3, SM 1007, SN 1207, CNFC 10408, CNCF 10429, LP 0403, and LP 0472). Experiment was composed of 4 blocks, and each plot with 5x5 m (diameter x width), being used spacing of 1 and 2 m among plots and blocks, respectively. Total soluble amino acids and proline were determined according to Peoples et al. (1989) and Bates et al. (1973), respectively. In this study were evaluated total soluble amino acids and proline. Data were analyzed employing a variance analysis, and using Scott-Knott test at 5% level of probability. Standard errors were also calculated in all treatments evaluated.

RESULTADOS:

Genotypes of common bean based in total soluble amino acids were described by two groups, in which group with higher level in this variable oscillated between 41.78 and 42.76 µmol g DM-1, being composed by genotypes IPR Uirapuru, CHC 9729, GEN C2-1-3, SM 1007, LP 0403, and LP 0472. In other group the genotypes IAPAR 81, IPR Tangará, BRS Campeiro, CHP 9859, GEN C2-1-1, CNFC 10408, and CNFC 10429 were statistically equals. Values obtained in proline indicate occurrence of three groups, being that group with greater proline accumulation presented as extremes values of 9.15 and 7.71 µmol g DM-1, and in this group the genotype that has higher proline level was IAPAR 81. However, genotypes that presented proline medium levels, if compared with other, was composed by IPR Uirapuru, CHC 9729, GEN C2-1-3, SM 1007, SN 1207, and CNFC 10429 genotypes. Results reported by investigating behavior of Vigna ungucuilata plants indicates that occurred proline accumulation under water deficiency, and results showed in this study can contribute in selection of tolerant genotypes to drought.

CONCLUSÃO:

Results indicated that IPR Uirapuru, CHC 9729, GEN C2-1-3, SM 1007, LP 0403, and LP 0472 genotypes presented higher total soluble amino acids. In addition, data linked to proline reveals that IAPAR 81 present high amount.

Palavras-chave: osmotic adjustment, proline, common bean.